Xn, Yn, Zn, λn, Tn
and the complete solution
Real-Time Super-Resolution Microscopy System
SRRF-Stream’ is a NEW real time super-resolution microscopy functionality that operates exclusively on Andor’s iXon Life and iXon Ultra EMCCD cameras.
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Real Time – enhanced workflow, avoids post-processing. View in ‘Live Mode’.
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Low Excitation Intensities (mW-W/cm2) – prolonged live cell observations and accurate physiology.
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Conventional Fluorophores, e.g. GFP – simple labelling, no photo-switching required.
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Live Cell Dynamics – full FOV super-res images every 1-2 secs. > 10 fps using ROI.
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Cost-Effective – convert conventional fluorescence microscopes to super-resolution microscopes.
Mitochondria
Clathrin Pits
SRRF vs SIM
Applications
Observational Capabilities of SRRF-Stream
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Elucidation of protein structure analysis at a sub-organelle level
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Tracking of single molecules inside cells
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Using this tracking to gain insight into individual molecular machinery underpinning cellular physiology
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With this new information, update models of cell function
Applications of SRRF-Stream
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Membrane fusion involving individual SNARE protein machinery
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Dynamic changes of and within synaptic vesicles
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Dendritic spines reformation due to synaptic plasticity and learning
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Signal transduction processes and cell-to-cell communication and differentiation
Microscopy for Multi-positions, Multi-channels, Z-stacks and Time-series acquisition.
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Olympus IX 83 Motorised with Z-focus Drive
XY Motorised Stages
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Mot. XY Stages (Märzhäuser, Applied Scientific Instrumentation)
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Piezo Z-Stage option
TTL Device Controller
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High-speed TTL Device Controller for hardware synchronization
Microscope Light Sources
and PC Workstation with Micro-Manager ver.1.4.xx Software
Citations
“SRRF: Universal live-cell super-resolution microscopy” (DOI 10.1016/j.biocel.2018.05.014)
Webinar
